Genotyping and primer details


Genotyping and primer details for SALK_128264c - At5g20500
Line ID SALK_128264c
Locus At5g20500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6938948 of line SALK_128264c Chr5:6938948
   
Insertion allele amplicon LBb3 - 37lm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37lm: 5'-TTTTGTTGAAGGAAAAGGAGTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6939529
   
wt amplicon (over InsPos) 04na - 37lm
wt amplicon start Chr5:6938666
wt amplicon end Chr5:6939529
wt amplicon size 864 bp
Locus specific primer 37lm: 5'-TTTTGTTGAAGGAAAAGGAGTGTT-3'
2nd locus specific primer 04na: 5'-ATCTATCTCCATGGTAATGCTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11