Genotyping and primer details


Genotyping and primer details for SALK_128432 - At3g58670
Line ID SALK_128432
Locus At3g58670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21705268 of line SALK_128432 Chr3:21705268
   
Insertion allele amplicon LBb3 - 64fr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 64fr: 5'-TCAATGAATTGAGAGAATTTGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21705770
   
wt amplicon (over InsPos) 08hi - 64fr
wt amplicon start Chr3:21704805
wt amplicon end Chr3:21705770
wt amplicon size 966 bp
Locus specific primer 64fr: 5'-TCAATGAATTGAGAGAATTTGCTG-3'
2nd locus specific primer 08hi: 5'-GAAAACTACATGCGATACCATCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11