Genotyping and primer details


Genotyping and primer details for SALK_130880c - At5g41090
Line ID SALK_130880c
Locus At5g41090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16446000 of line SALK_130880c Chr5:16446000
   
Insertion allele amplicon LBb3 - 28lu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28lu: 5'-TCATGAAGAAGCTAAGGTGTAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16446548
   
wt amplicon (over InsPos) 18mu - 28lu
wt amplicon start Chr5:16445521
wt amplicon end Chr5:16446548
wt amplicon size 1028 bp
Locus specific primer 28lu: 5'-TCATGAAGAAGCTAAGGTGTAGCA-3'
2nd locus specific primer 18mu: 5'-CGTGGCGTGATATTATCTATTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11