Genotyping and primer details


Genotyping and primer details for SALK_133064c - At1g66020
Line ID SALK_133064c
Locus At1g66020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24579792 of line SALK_133064c Chr1:24579792
   
Insertion allele amplicon R212 - 38xo
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 38xo: 5'-GTCAAATATTCACGGTATTCTCGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24580654
   
Insertion allele amplicon LBb3 - CG21
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG21: 5'-GTAGTGGTGGTCATGATGAAAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24578997
   
wt amplicon (over InsPos) CG22 - CG21
wt amplicon start Chr1:24578997
wt amplicon end Chr1:24579904
wt amplicon size 908 bp
Locus specific primer CG21: 5'-GTAGTGGTGGTCATGATGAAAATG-3'
2nd locus specific primer CG22: 5'-GAAGATCATACGTGCAAGATCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11