Genotyping and primer details


Genotyping and primer details for SALK_134125c - At5g06910
Line ID SALK_134125c
Locus At5g06910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2140467 of line SALK_134125c Chr5:2140467
   
Insertion allele amplicon LBb3 - 13gj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13gj: 5'-TTTCTTATTTCCTGTGAAGTGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2141047
   
wt amplicon (over InsPos) 13ho - 13gj
wt amplicon start Chr5:2140115
wt amplicon end Chr5:2141047
wt amplicon size 933 bp
Locus specific primer 13gj: 5'-TTTCTTATTTCCTGTGAAGTGGCT-3'
2nd locus specific primer 13ho: 5'-TTATTGTGACTGGGATCAAACAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11