Genotyping and primer details


Genotyping and primer details for SALK_135697c - At3g13430
Line ID SALK_135697c
Locus At3g13430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4368586 of line SALK_135697c Chr3:4368586
   
Insertion allele amplicon LBb3 - 32td
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 32td: 5'-AAATTGAAGCAAACCAGAAAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4368899
   
wt amplicon (over InsPos) 15ua - 32td
wt amplicon start Chr3:4368280
wt amplicon end Chr3:4368899
wt amplicon size 620 bp
Locus specific primer 32td: 5'-AAATTGAAGCAAACCAGAAAGAAA-3'
2nd locus specific primer 15ua: 5'-GTGACTATTTCATTGGACCTGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11