Genotyping and primer details


Genotyping and primer details for SALK_136073c - At1g49490
Line ID SALK_136073c
Locus At1g49490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18319189 of line SALK_136073c Chr1:18319189
   
Insertion allele amplicon LBb3 - 38mv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38mv: 5'-TAGATGATGATAGTCTCACGGTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18319805
   
wt amplicon (over InsPos) 20oj - 38mv
wt amplicon start Chr1:18318708
wt amplicon end Chr1:18319805
wt amplicon size 1098 bp
Locus specific primer 38mv: 5'-TAGATGATGATAGTCTCACGGTCG-3'
2nd locus specific primer 20oj: 5'-TAGACGGTTGTGGTTTAGGAGACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11