Genotyping and primer details


Genotyping and primer details for SALK_137069 - At2g32510
Line ID SALK_137069
Locus At2g32510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13798902 of line SALK_137069 Chr2:13798902
   
Insertion allele amplicon LBb3 - 41cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 41cv: 5'-TGATAGAGATGGTTACGGGTTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13799374
   
wt amplicon (over InsPos) 33dz - 41cv
wt amplicon start Chr2:13798322
wt amplicon end Chr2:13799374
wt amplicon size 1053 bp
Locus specific primer 41cv: 5'-TGATAGAGATGGTTACGGGTTCTC-3'
2nd locus specific primer 33dz: 5'-TTCTCATTTTCCCCACAAGTTTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11