Genotyping and primer details


Genotyping and primer details for SALK_137266c - At4g09900
Line ID SALK_137266c
Locus At4g09900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:6223563 of line SALK_137266c Chr4:6223563
   
Insertion allele amplicon LBb3 - 22gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22gq: 5'-TTAAAGTCCACTTCTTTGGGCTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6223967
   
wt amplicon (over InsPos) 19ir - 22gq
wt amplicon start Chr4:6223024
wt amplicon end Chr4:6223967
wt amplicon size 944 bp
Locus specific primer 22gq: 5'-TTAAAGTCCACTTCTTTGGGCTAA-3'
2nd locus specific primer 19ir: 5'-TCCAAAAACTCAGGAACCTAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11