Genotyping and primer details


Genotyping and primer details for SALK_137617c - At5g02840
Line ID SALK_137617c
Locus At5g02840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:649152 of line SALK_137617c Chr5:649152
   
Insertion allele amplicon LBb3 - 67ns
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 67ns: 5'-GGATATCGTCGTCATTTCTCTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:648706
   
wt amplicon (over InsPos) 08or - 67ns
wt amplicon start Chr5:648706
wt amplicon end Chr5:649554
wt amplicon size 849 bp
Locus specific primer 67ns: 5'-GGATATCGTCGTCATTTCTCTCTC-3'
2nd locus specific primer 08or: 5'-AAGAGAGAGAAGCCTTTCACACAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11