Genotyping and primer details


Genotyping and primer details for SALK_138548c - At3g07510
Line ID SALK_138548c
Locus At3g07510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2393992 of line SALK_138548c Chr3:2393992
   
Insertion allele amplicon LBb3 - 94yp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 94yp: 5'-AGGGTTTGGTTAGGTATTCGGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2392828
   
Insertion allele amplicon R204 - BL15
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BL15: 5'-TGAGTAAGCAAACCCTCTCTCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2394570
   
wt amplicon (over InsPos) BL16 - BL15
wt amplicon start Chr3:2393489
wt amplicon end Chr3:2394570
wt amplicon size 1082 bp
Locus specific primer BL15: 5'-TGAGTAAGCAAACCCTCTCTCTTC-3'
2nd locus specific primer BL16: 5'-TGTACAAATGAACTTTTCAGGACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11