Genotyping and primer details


Genotyping and primer details for SALK_138624 - At1g17950
Line ID SALK_138624
Locus At1g17950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6179042 of line SALK_138624 Chr1:6179042
   
Insertion allele amplicon LBb3 - 02bz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 02bz: 5'-CAAGTCCTCAAAGAGTTCTTGACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6178361
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11