Genotyping and primer details


Genotyping and primer details for SALK_139161 - At5g45770
Line ID SALK_139161
Locus At5g45770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18564859 of line SALK_139161 Chr5:18564859
   
Insertion allele amplicon LBb3 - 46wj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46wj: 5'-ATCTCACTGGCCTTATCCCTAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18564112
   
wt amplicon (over InsPos) 10xf - 46wj
wt amplicon start Chr5:18564112
wt amplicon end Chr5:18565720
wt amplicon size 1609 bp
Locus specific primer 46wj: 5'-ATCTCACTGGCCTTATCCCTAAAT-3'
2nd locus specific primer 10xf: 5'-TTGTGAGGTTTAGTCAGAATGCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11