Genotyping and primer details


Genotyping and primer details for SALK_139620 - At1g56690
Line ID SALK_139620
Locus At1g56690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:21255798 of line SALK_139620 Chr1:21255798
   
Insertion allele amplicon LBb3 - 27de
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27de: 5'-GGTTTTCCTCAAAGGACATTATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21254988
   
wt amplicon (over InsPos) 34dz - 27de
wt amplicon start Chr1:21254988
wt amplicon end Chr1:21256053
wt amplicon size 1066 bp
Locus specific primer 27de: 5'-GGTTTTCCTCAAAGGACATTATCA-3'
2nd locus specific primer 34dz: 5'-ATTGAGTGGTGCATTGATATGTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11