Genotyping and primer details


Genotyping and primer details for SALK_141756c - At1g28440
Line ID SALK_141756c
Locus At1g28440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9999751 of line SALK_141756c Chr1:9999751
   
Insertion allele amplicon LBb3 - 28fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28fx: 5'-GGCTATGTATGGCTTCTTAGATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9998759
   
wt amplicon (over InsPos) 22lt - 28fx
wt amplicon start Chr1:9998759
wt amplicon end Chr1:9999997
wt amplicon size 1239 bp
Locus specific primer 28fx: 5'-GGCTATGTATGGCTTCTTAGATCG-3'
2nd locus specific primer 22lt: 5'-GAGTCCAACGTTGAGGATTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11