Genotyping and primer details


Genotyping and primer details for SALK_142729 - At4g14760
Line ID SALK_142729
Locus At4g14760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8478380 of line SALK_142729 Chr4:8478380
   
Insertion allele amplicon R204 - CE25
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CE25: 5'-AGTGCTTTGATGATTTCTTCCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8477971
   
Insertion allele amplicon LBb3 - CE26
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CE26: 5'-GTCCAAGTAGAGATAAAAGCACCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8478714
   
wt amplicon (over InsPos) CE26 - CE25
wt amplicon start Chr4:8477971
wt amplicon end Chr4:8478714
wt amplicon size 744 bp
Locus specific primer CE25: 5'-AGTGCTTTGATGATTTCTTCCAGT-3'
2nd locus specific primer CE26: 5'-GTCCAAGTAGAGATAAAAGCACCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11