Genotyping and primer details


Genotyping and primer details for SALK_143623c - At2g41420
Line ID SALK_143623c
Locus At2g41420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17267385 of line SALK_143623c Chr2:17267385
   
Insertion allele amplicon LBb3 - 15ew
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15ew: 5'-ATCATTGAGATGTGACCATACGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17266493
   
wt amplicon (over InsPos) 21lt - 15ew
wt amplicon start Chr2:17266493
wt amplicon end Chr2:17267717
wt amplicon size 1225 bp
Locus specific primer 15ew: 5'-ATCATTGAGATGTGACCATACGAA-3'
2nd locus specific primer 21lt: 5'-TCTGAATTTTGTTTGTGCTGAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11