Genotyping and primer details


Genotyping and primer details for SALK_143628c - At3g03780
Line ID SALK_143628c
Locus At3g03780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:960448 of line SALK_143628c Chr3:960448
   
Insertion allele amplicon LBb3 - 23gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23gq: 5'-AACACAAACCACGTAGAAAAATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:960794
   
wt amplicon (over InsPos) 06hv - 23gq
wt amplicon start Chr3:960054
wt amplicon end Chr3:960794
wt amplicon size 741 bp
Locus specific primer 23gq: 5'-AACACAAACCACGTAGAAAAATCC-3'
2nd locus specific primer 06hv: 5'-CAATTCTCAACTGGTCTTTTGTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11