Genotyping and primer details


Genotyping and primer details for SALK_143700c - At2g37050
Line ID SALK_143700c
Locus At2g37050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15570599 of line SALK_143700c Chr2:15570599
   
Insertion allele amplicon R204 - 07as
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 07as: 5'-TCCCTGCTGATTCAAGAAAGTATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15569771
   
wt amplicon (over InsPos) 03bs - 07as
wt amplicon start Chr2:15569771
wt amplicon end Chr2:15570754
wt amplicon size 984 bp
Locus specific primer 07as: 5'-TCCCTGCTGATTCAAGAAAGTATT-3'
2nd locus specific primer 03bs: 5'-TACTCACCATCTACTGAACCATCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11