Genotyping and primer details


Genotyping and primer details for SALK_144605c - At4g29000
Line ID SALK_144605c
Locus At4g29000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14295096 of line SALK_144605c Chr4:14295096
   
Insertion allele amplicon LBb3 - 24kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 24kh: 5'-TATCTGCTAAAAGGGACCTTCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14295713
   
wt amplicon (over InsPos) 11mc - 24kh
wt amplicon start Chr4:14294561
wt amplicon end Chr4:14295713
wt amplicon size 1153 bp
Locus specific primer 24kh: 5'-TATCTGCTAAAAGGGACCTTCAAA-3'
2nd locus specific primer 11mc: 5'-CTGCTTGAAATTGTAAGTCTTCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11