Genotyping and primer details

Genotyping and primer details for SALK_145146 - At2g46260
Line ID SALK_145146
Locus At2g46260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18996537 of line SALK_145146 Chr2:18996537
Insertion allele amplicon LBb3 - 09bt
Locus specific primer 09bt: 5'-TTGTCGTACCTTGTGCACTAATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18997055
wt amplicon (over InsPos) 28da - 09bt
wt amplicon start Chr2:18996024
wt amplicon end Chr2:18997055
wt amplicon size 1032 bp
Locus specific primer 09bt: 5'-TTGTCGTACCTTGTGCACTAATTC-3'
2nd locus specific primer 28da: 5'-CGAATTTGACCCTTATTGTTATCC-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11