Genotyping and primer details


Genotyping and primer details for SALK_147032c - At3g19380
Line ID SALK_147032c
Locus At3g19380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6714620 of line SALK_147032c Chr3:6714620
   
Insertion allele amplicon LBb3 - 57cy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 57cy: 5'-GCCCAGTTTGTTTATTGTTCATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6713969
   
wt amplicon (over InsPos) 36dz - 57cy
wt amplicon start Chr3:6713969
wt amplicon end Chr3:6715095
wt amplicon size 1127 bp
Locus specific primer 57cy: 5'-GCCCAGTTTGTTTATTGTTCATTT-3'
2nd locus specific primer 36dz: 5'-CTCTCAAAATCGGAATCAAAACTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11