Genotyping and primer details


Genotyping and primer details for SALK_147407 - At1g52550
Line ID SALK_147407
Locus At1g52550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19573162 of line SALK_147407 Chr1:19573162
   
Insertion allele amplicon LBb3 - 01cb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 01cb: 5'-AGCACACACCAAACACATCTAGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19572887
   
Insertion allele amplicon LBb3 - 06cq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 06cq: 5'-TGAAGGTGGTTTAACTGCTATTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19573623
   
Insertion allele amplicon LBb3 - 16wp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16wp: 5'-ACCTCACCTTGGTGAATCTGTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19572339
   
Insertion allele amplicon LBb3 - SC04
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer SC04: 5'-TCAAGATCGATTACTGTTCATTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19573570
   
wt amplicon (over InsPos) 01cb - 06cq
wt amplicon start Chr1:19572887
wt amplicon end Chr1:19573623
wt amplicon size 737 bp
Locus specific primer 06cq: 5'-TGAAGGTGGTTTAACTGCTATTGA-3'
2nd locus specific primer 01cb: 5'-AGCACACACCAAACACATCTAGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11