Genotyping and primer details


Genotyping and primer details for SALK_147747c - At3g57220
Line ID SALK_147747c
Locus At3g57220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21174316 of line SALK_147747c Chr3:21174316
   
Insertion allele amplicon R212 - 07uk
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 07uk: 5'-GGAATCAACTTGAGTGTGACAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21174799
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11