Genotyping and primer details


Genotyping and primer details for SALK_148352c - At2g02080
Line ID SALK_148352c
Locus At2g02080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:520658 of line SALK_148352c Chr2:520658
   
Insertion allele amplicon LBb3 - 29rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29rb: 5'-GATTCAACAACCTAACTCCTCAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:521045
   
wt amplicon (over InsPos) 45sq - 29rb
wt amplicon start Chr2:519804
wt amplicon end Chr2:521045
wt amplicon size 1242 bp
Locus specific primer 29rb: 5'-GATTCAACAACCTAACTCCTCAGC-3'
2nd locus specific primer 45sq: 5'-CAACTCATGCATGTGAGTATGTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11