Genotyping and primer details

Genotyping and primer details for SALK_149927c - At1g12460
Line ID SALK_149927c
Locus At1g12460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4248587 of line SALK_149927c Chr1:4248587
Insertion allele amplicon LBb3 - 66cs
Locus specific primer 66cs: 5'-AAATGCAGCTACTTGTTCTTGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4247570
wt amplicon (over InsPos) 18lt - 66cs
wt amplicon start Chr1:4247570
wt amplicon end Chr1:4249101
wt amplicon size 1532 bp
Locus specific primer 66cs: 5'-AAATGCAGCTACTTGTTCTTGGTT-3'
2nd locus specific primer 18lt: 5'-AAAGATCAAGAAACTGGACTTTGG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11