Genotyping and primer details


Genotyping and primer details for SALK_150636c - At3g58170
Line ID SALK_150636c
Locus At3g58170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21543776 of line SALK_150636c Chr3:21543776
   
Insertion allele amplicon LBb3 - 30rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30rb: 5'-CAAGTTAACCATCGATGAAACTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21544227
   
wt amplicon (over InsPos) 28rz - 30rb
wt amplicon start Chr3:21543298
wt amplicon end Chr3:21544227
wt amplicon size 930 bp
Locus specific primer 30rb: 5'-CAAGTTAACCATCGATGAAACTGA-3'
2nd locus specific primer 28rz: 5'-ACACAAGTTTGAACATGACAGAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11