Genotyping and primer details


Genotyping and primer details for SALK_151204 - At1g18840
Line ID SALK_151204
Locus At1g18840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6502608 of line SALK_151204 Chr1:6502608
   
Insertion allele amplicon LBb3 - 55xo
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 55xo: 5'-CCATAATATTTTGTGATTGTTCGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6503496
   
Insertion allele amplicon R204 - AW49
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW49: 5'-CCTCGACTATCTGAGGATTACTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6502048
   
wt amplicon (over InsPos) AW50 - AW49
wt amplicon start Chr1:6502048
wt amplicon end Chr1:6502902
wt amplicon size 855 bp
Locus specific primer AW49: 5'-CCTCGACTATCTGAGGATTACTGG-3'
2nd locus specific primer AW50: 5'-CAGATTCTCAAGATGTTGCTTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11