Genotyping and primer details

Genotyping and primer details for SALK_151293c - At4g07390
Line ID SALK_151293c
Locus At4g07390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:4196810 of line SALK_151293c Chr4:4196810
Insertion allele amplicon LBb3 - 51wj
Locus specific primer 51wj: 5'-TACCTTTGCAGCTTTAATCTTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:4196195
wt amplicon (over InsPos) 05wy - 51wj
wt amplicon start Chr4:4196195
wt amplicon end Chr4:4197586
wt amplicon size 1392 bp
Locus specific primer 51wj: 5'-TACCTTTGCAGCTTTAATCTTGGT-3'
2nd locus specific primer 05wy: 5'-CCATCAGATTTGGATCTTGGTAAT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11