Genotyping and primer details


Genotyping and primer details for 809D12 - At1g18910
Line ID 809D12
Locus At1g18910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6533228 of line 809D12 Chr1:6533228
   
Insertion allele amplicon 3144 - AF10
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AF10: 5'-AACTGGAGTAGTTTTTGACGGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6533578
   
Insertion allele amplicon 8474 - GA44
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GA44: 5'-CCTTTCTTCCACAGTCATTACACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6532777
   
wt amplicon (over InsPos) AF10 - GA44
wt amplicon start Chr1:6532777
wt amplicon end Chr1:6533578
wt amplicon size 802 bp
Locus specific primer GA44: 5'-CCTTTCTTCCACAGTCATTACACA-3'
2nd locus specific primer AF10: 5'-AACTGGAGTAGTTTTTGACGGCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11