Genotyping and primer details


Genotyping and primer details for SALK_035172 - At1g26620
Line ID SALK_035172
Locus At1g26620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9195984 of line SALK_035172 Chr1:9195984
   
Insertion allele amplicon LBb3 - 16hd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16hd: 5'-AGAAGCAATAGTTGCCATCTCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9196531
   
wt amplicon (over InsPos) 06il - 16hd
wt amplicon start Chr1:9195558
wt amplicon end Chr1:9196531
wt amplicon size 974 bp
Locus specific primer 16hd: 5'-AGAAGCAATAGTTGCCATCTCAAT-3'
2nd locus specific primer 06il: 5'-ATGGACAATCGTTTTATACTTGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11