Genotyping and primer details


Genotyping and primer details for 718B05 - At1g67110
Line ID 718B05
Locus At1g67110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25063175 of line 718B05 Chr1:25063175
   
Insertion allele amplicon 8474 - 56lm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 56lm: 5'-AAAGCCAATTCATCATGAGTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25062548
   
wt amplicon (over InsPos) 07mu - 56lm
wt amplicon start Chr1:25062548
wt amplicon end Chr1:25063694
wt amplicon size 1147 bp
Locus specific primer 56lm: 5'-AAAGCCAATTCATCATGAGTCTCT-3'
2nd locus specific primer 07mu: 5'-AACAGCTCTCAAGTCTTACTTCGG-3'
   
Note

Last Updated on Thursday, 06 December 2018 16:11