Genotyping and primer details


Genotyping and primer details for SALK_150241c - At2g30290
Line ID SALK_150241c
Locus At2g30290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12912923 of line SALK_150241c Chr2:12912923
   
Insertion allele amplicon LBb3 - 29eb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29eb: 5'-ATGTGAATGAGTGTGAGGAGAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12913383
   
Insertion allele amplicon LBb3 - CF81
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CF81: 5'-GTGTAGAGAAAAGCGATTTTGGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12912354
   
wt amplicon (over InsPos) CF81 - CF80
wt amplicon start Chr2:12912354
wt amplicon end Chr2:12913384
wt amplicon size 1031 bp
Locus specific primer CF80: 5'-GATGTGAATGAGTGTGAGGAGAAA-3'
2nd locus specific primer CF81: 5'-GTGTAGAGAAAAGCGATTTTGGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11