Genotyping and primer details


Genotyping and primer details for 162B10 - At3g18350
Line ID 162B10
Locus At3g18350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6299835 of line 162B10 Chr3:6299835
   
Insertion allele amplicon 8474 - 02us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02us: 5'-CACTCTCTCAACCGCCTTATTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6300430
   
wt amplicon (over InsPos) 71xr - 02us
wt amplicon start Chr3:6298594
wt amplicon end Chr3:6300430
wt amplicon size 1837 bp
Locus specific primer 02us: 5'-CACTCTCTCAACCGCCTTATTAAC-3'
2nd locus specific primer 71xr: 5'-ATTCTGGAAATAGACGGGACAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11