Genotyping and primer details


Genotyping and primer details for 002A10 - At3g14310
Line ID 002A10
Locus At3g14310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4774933 of line 002A10 Chr3:4774933
   
Insertion allele amplicon 8474 - TH24
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer TH24: 5'-CAAGGCTTTACGAACTTGCTTATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4774511
   
wt amplicon (over InsPos) 07bs - TH24
wt amplicon start Chr3:4774511
wt amplicon end Chr3:4775434
wt amplicon size 924 bp
Locus specific primer TH24: 5'-CAAGGCTTTACGAACTTGCTTATC-3'
2nd locus specific primer 07bs: 5'-GGCAAAGCAACGAATATTTAGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11