Genotyping and primer details


Genotyping and primer details for 008H11 - At3g01310
Line ID 008H11
Locus At3g01310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:98723 of line 008H11 Chr3:98723
   
Insertion allele amplicon 8474 - MG03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MG03: 5'-AAGAAAACTTCAACGGGATAGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:98193
   
wt amplicon (over InsPos) 10sl - MG03
wt amplicon start Chr3:98193
wt amplicon end Chr3:99083
wt amplicon size 891 bp
Locus specific primer MG03: 5'-AAGAAAACTTCAACGGGATAGACA-3'
2nd locus specific primer 10sl: 5'-GGTTTGATCTTCTACGATCTGAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11