Genotyping and primer details


Genotyping and primer details for 012H03 - At1g25270
Line ID 012H03
Locus At1g25270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8859736 of line 012H03 Chr1:8859736
   
Insertion allele amplicon 3144 - 02cp
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 02cp: 5'-AAACATTTTGTTGAGTTCCAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8860298
   
wt amplicon (over InsPos) 04dn - 02cp
wt amplicon start Chr1:8859289
wt amplicon end Chr1:8860298
wt amplicon size 1010 bp
Locus specific primer 02cp: 5'-AAACATTTTGTTGAGTTCCAAACC-3'
2nd locus specific primer 04dn: 5'-CAAGGAACTTGGAGGACTTTATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11