Genotyping and primer details


Genotyping and primer details for 014A07 - At3g06820
Line ID 014A07
Locus At3g06820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2151355 of line 014A07 Chr3:2151355
   
Insertion allele amplicon 8474 - KU42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KU42: 5'-GAAATATACACAAAGAAGACGGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2150979
   
wt amplicon (over InsPos) 65rd - KU42
wt amplicon start Chr3:2150979
wt amplicon end Chr3:2151778
wt amplicon size 800 bp
Locus specific primer KU42: 5'-GAAATATACACAAAGAAGACGGGC-3'
2nd locus specific primer 65rd: 5'-CTCCGTTCAAATTTAGATACCAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11