Genotyping and primer details


Genotyping and primer details for 016A11 - At2g23540
Line ID 016A11
Locus At2g23540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10025575 of line 016A11 Chr2:10025575
   
Insertion allele amplicon 8474 - 01kq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01kq: 5'-TTCTTCAACACTACACGGAAACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10025138
   
wt amplicon (over InsPos) 66rd - 01kq
wt amplicon start Chr2:10025138
wt amplicon end Chr2:10026011
wt amplicon size 874 bp
Locus specific primer 01kq: 5'-TTCTTCAACACTACACGGAAACAG-3'
2nd locus specific primer 66rd: 5'-CATCACCATATAACAATTGCTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11