Genotyping and primer details


Genotyping and primer details for 018D02 - At1g77310
Line ID 018D02
Locus At1g77310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29053261 of line 018D02 Chr1:29053261
   
Insertion allele amplicon 8474 - 01dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01dl: 5'-GCGTGAACTCTCCTAATTAAATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29053814
   
wt amplicon (over InsPos) 08ec - 01dl
wt amplicon start Chr1:29052678
wt amplicon end Chr1:29053814
wt amplicon size 1137 bp
Locus specific primer 01dl: 5'-GCGTGAACTCTCCTAATTAAATGC-3'
2nd locus specific primer 08ec: 5'-TCCTCTTTTTGTTGTATGTTGTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11