Genotyping and primer details


Genotyping and primer details for 020E07 - At3g44150
Line ID 020E07
Locus At3g44150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:15887983 of line 020E07 Chr3:15887983
   
Insertion allele amplicon 8474 - 04uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04uv: 5'-ACTATCTATTCACCATGCTCCGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:15887713
   
wt amplicon (over InsPos) 54vd - 04uv
wt amplicon start Chr3:15887713
wt amplicon end Chr3:15888833
wt amplicon size 1121 bp
Locus specific primer 04uv: 5'-ACTATCTATTCACCATGCTCCGAC-3'
2nd locus specific primer 54vd: 5'-TAACTATTTCCGCAATTTCGTGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11