Genotyping and primer details


Genotyping and primer details for 021F05 - At1g44318
Line ID 021F05
Locus At1g44318 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:16832721 of line 021F05 Chr1:16832721
   
Insertion allele amplicon 8474 - DC62
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DC62: 5'-TGGTCAATAGCTAGAGGAAGTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16832190
   
wt amplicon (over InsPos) 11sl - DC62
wt amplicon start Chr1:16832190
wt amplicon end Chr1:16833175
wt amplicon size 986 bp
Locus specific primer DC62: 5'-TGGTCAATAGCTAGAGGAAGTGGT-3'
2nd locus specific primer 11sl: 5'-GGCGTGTGCAAATTAATAAAGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11