Genotyping and primer details


Genotyping and primer details for 025E12 - At4g28370
Line ID 025E12
Locus At4g28370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14037821 of line 025E12 Chr4:14037821
   
Insertion allele amplicon 8474 - 02vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02vh: 5'-ATGAAACATCAGCATTAGCATCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14038297
   
wt amplicon (over InsPos) 50vx - 02vh
wt amplicon start Chr4:14036989
wt amplicon end Chr4:14038297
wt amplicon size 1309 bp
Locus specific primer 02vh: 5'-ATGAAACATCAGCATTAGCATCTG-3'
2nd locus specific primer 50vx: 5'-TGTTTTTCCCCTTTGTTACTGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11