Genotyping and primer details


Genotyping and primer details for 025H01 - At2g31010
Line ID 025H01
Locus At2g31010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13197540 of line 025H01 Chr2:13197540
   
Insertion allele amplicon 8474 - 01ie
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01ie: 5'-TTGAGCACTGGTAGGAATTCTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13196953
   
wt amplicon (over InsPos) 03jf - 01ie
wt amplicon start Chr2:13196953
wt amplicon end Chr2:13197913
wt amplicon size 961 bp
Locus specific primer 01ie: 5'-TTGAGCACTGGTAGGAATTCTTTT-3'
2nd locus specific primer 03jf: 5'-TGAGAAAGCACAAACAAAAATCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11