Genotyping and primer details


Genotyping and primer details for 027C03 - At1g73160
Line ID 027C03
Locus At1g73160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27507074 of line 027C03 Chr1:27507074
   
Insertion allele amplicon 8474 - 01jr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01jr: 5'-GGCTCTAAAGCTCCTAAAACCCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27507612
   
wt amplicon (over InsPos) 04km - 01jr
wt amplicon start Chr1:27506667
wt amplicon end Chr1:27507612
wt amplicon size 946 bp
Locus specific primer 01jr: 5'-GGCTCTAAAGCTCCTAAAACCCTA-3'
2nd locus specific primer 04km: 5'-TTGATTACTCTCCGGTGACTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11