Genotyping and primer details


Genotyping and primer details for 031H11 - At3g59290
Line ID 031H11
Locus At3g59290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21914647 of line 031H11 Chr3:21914647
   
Insertion allele amplicon 8474 - 02vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02vd: 5'-TGATTCATTAACAAAATCAGCAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21915089
   
wt amplicon (over InsPos) 70vs - 02vd
wt amplicon start Chr3:21914098
wt amplicon end Chr3:21915089
wt amplicon size 992 bp
Locus specific primer 02vd: 5'-TGATTCATTAACAAAATCAGCAGC-3'
2nd locus specific primer 70vs: 5'-AGCACATATTTCCCATAACCTGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11