Genotyping and primer details


Genotyping and primer details for 032D02 - At3g44970
Line ID 032D02
Locus At3g44970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16433774 of line 032D02 Chr3:16433774
   
Insertion allele amplicon 8474 - CE01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CE01: 5'-TTCTTTCCTTAGGAGTCACGGTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16433345
   
wt amplicon (over InsPos) CE02 - CE01
wt amplicon start Chr3:16433345
wt amplicon end Chr3:16434247
wt amplicon size 903 bp
Locus specific primer CE01: 5'-TTCTTTCCTTAGGAGTCACGGTAA-3'
2nd locus specific primer CE02: 5'-GATTCCACAAAAACGTCAAATTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11