Genotyping and primer details


Genotyping and primer details for 033G12 - At2g25310
Line ID 033G12
Locus At2g25310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10778820 of line 033G12 Chr2:10778820
   
Insertion allele amplicon 8474 - 02kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02kh: 5'-ATTTTGGGGTGAAAATGTAGACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10779434
   
wt amplicon (over InsPos) 07ky - 02kh
wt amplicon start Chr2:10778509
wt amplicon end Chr2:10779434
wt amplicon size 926 bp
Locus specific primer 02kh: 5'-ATTTTGGGGTGAAAATGTAGACTG-3'
2nd locus specific primer 07ky: 5'-CCTGAGAAACGTAATCTTCTGTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11