Genotyping and primer details


Genotyping and primer details for 035G08 - At1g09575
Line ID 035G08
Locus At1g09575 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3102073 of line 035G08 Chr1:3102073
   
Insertion allele amplicon 8474 - 06uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06uv: 5'-CTAATGAGGCTTGTGAATGTTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3102661
   
wt amplicon (over InsPos) 09vj - 06uv
wt amplicon start Chr1:3101753
wt amplicon end Chr1:3102661
wt amplicon size 909 bp
Locus specific primer 06uv: 5'-CTAATGAGGCTTGTGAATGTTGAA-3'
2nd locus specific primer 09vj: 5'-ACGGAAAACAATCTTTGTCACTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11