Genotyping and primer details


Genotyping and primer details for 039A04 - At3g61420
Line ID 039A04
Locus At3g61420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22727305 of line 039A04 Chr3:22727305
   
Insertion allele amplicon 8474 - 02ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02ra: 5'-TGACCGATTCAGTGACTACATAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22726947
   
wt amplicon (over InsPos) 48si - 02ra
wt amplicon start Chr3:22726947
wt amplicon end Chr3:22727588
wt amplicon size 642 bp
Locus specific primer 02ra: 5'-TGACCGATTCAGTGACTACATAGC-3'
2nd locus specific primer 48si: 5'-ATGGTCTAGTGTTTATGGTTTCCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11